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secondary antibody review -- data from 99 publications

cytometry   used as a control to detect cell responses targeted antigen   7       Alexa Fluor 488         7       Cy3         8 goat IgG   Alexa Fluor 488   1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey

In situ hybridization of mammalian cells (RNA and Oligonucleotide probes)

in the hybridization mixture.VI. Antibody detection (optional): Digoxigenin-labeled probes can be detected with sheep anti-digoxigenin antibodies (1/200, Boehringer Mannheim), and then with donkey anti-sheep antibodies conjugated to fluorescein (1/150, Sigma). Slides

RNA Whole Mount In Situ Hybridization

a dilution of 1:5000 to 1:10,000). For chick embryos, put roughly 3 mg of chick embryo powder into a microtube with 0.5 ml TBST. Heat at 70° C for 30 minutes, vortex for 10 minutes. Cool on ice, add ml sheep serum and 1 m l anti-dig AP antibody. Shake