sigma-aldrich特约代理商|D8938-1SE D

Preparation of Tonsil Lysate

more than the volume stated on the side of the dounce homogenizer. When you are done transfer homogenized tissue to a 1 liter bottle and add in the stir bar. Bring the volume up to 1 liter by adding more of the Lysis buffer. Add Triton-X 100 to a final concentration of 2%

RNA Extraction--RNA提取

tubes in a beaker of DEPC water, or fill with DEPC water and place on nutator O/N, follwed by autoclaving.Keep RNA prep on ice unless otherwise indicated.1. Grind tissue wit baked mortor and pestle in liquid nitrogen. (5g of tissue

Myosin Light Chain Preparation

into strips and run through the meat grinder.Approximately 750 grams of tissue is needed. 2.Tissue is weighed (900 g). 3.The tissue is homogenized in 6 volumes of Buffer A using a large blender and blending for a total time of 90 seconds.It is best to bland