- ￥3000
- sigma-aldrich
- D8938-1SE
- 2025年07月08日
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- 详细信息
- 文献和实验
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- 供应商：
上海玉博生物科技有限公司
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文献和实验Western Blot/Tissue Preparation
. Dice into very small pieces using a clean razor blade. Add 3 ml of ice cold RIPA buffer (supplemented with protease inhibitors) per gram of tissue. Further disrupt and homogenize tissue with a dounce homogenizer or a sonicator
for the first time add 10 µL of ßME per mL of Buffer RLT, and add 4 vol of EtOH to buffer RPE. 2) For tissues: Mince tissue with a scalpel and then dounce 50 - 250 µg of tissue in 2 mL of Buffer RLT. Tranfer to a sterile 15 mL conical tube. Wash the dounce
1. Snap freeze ~107 cells or ~100 mg of tissue in liquid nitrogen or dry ice/ethanol.2. Transfer the frozen sample to a mortar and pestle and grind to a fine powder. Or use a hand-held tissue grinder for a microcentrifuge tube.3. Transfer the sample
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