GE特约代理商|17-0609-01 试剂SEPHACRYL

Phosphoamino acid analysis:Mark Kamps's method

or enough to submerge your piece of membrane. 3.Incubate in 110℃ oven for 60 min. 4.Microfuge sample full speed for at least 1 min. 5.Transfer supernatant to new tube and lyophilize on Speedivac.(It takes about 3 hr.) 6.Resuspend in H2O,and microfuge 5 min.Spotting

MHC-I单体的S300 柱层析

Collector，Pharmacia) （4）层析记录仪（Chart Recorder， Pharmacia) （5）层析柱（Hiprep 26/60 Sephacryl S-400 Size exclusion column， Pharmacia) 2、   试剂       层析缓冲液：20mM Tris(PH8.0)；150mM NaCl （4 o C预冷）   【操作步骤】 （1）准备好足够量的层析缓冲液，按照操作程序接好层析装置，将进样口与出样

Kamps's Western Blotting Protocol

antibodies,we use 2 micrograms/ml.The origninal lab protocol called for a 2 hr incubation,but that seems unnecessary. 7)Wash the filter 2 x 5 min with rinsing buffer 1 x 5 min with NP40 buffer then 2 x 5 min with rinsing buffer. With anti-phosphotyrosine