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上海玉博生物科技有限公司
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文献和实验Plasmid Preparation on Sephacryl S1000
, and (5) recovery of plasmid DNA band (extraction of ethidium bromide and cesium chloride). Steps (4) and (5) of this procedure are expensive, time-consuming, and require expensive machinery [ultracentrifuge, rotor(s)]. In addition, incomplete extraction of ethidium
MHC-I 单体的S300 柱层析 在上述生物素化的样品中,折叠为单体的HLA-I类分子相当于样品中S300的组分。经过S300柱层析,可从样品中分离出折叠为单体的HLA-I类分子 【试剂及材料】 1、 材料 (1)层析蠕动泵(Hiload Pump P-50,Pharmacia) (2)紫外监视仪(Monitor UV-1,Pharmacia) (3)部分收集器(RediFrac Fraction
Vybrant® DyeCycle Green and Orange Stains
stain is used in combination with other stains for multicolor applications, apply the other stain(s) to the sample first, following all manufacturers’ instructions, including wash steps. The Vybrant® DyeCycle™ stain should be the last stain applied
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