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文献和实验Assembly of Nucleosomal Templates by Salt Dialysis
8.0 ( appendix 22 ) 10,000 U/ml T4 polynucleotide kinase (Promega) and 10× T4 polynucleotide kinase buffer 10 µCi/µl [γ ‐32 P]dATP
posttranslational modifications of histones, and the cumbersome use of reverse-phase high-performance liquid chromatography. The three methodologies are shorter than the conventionally used protocols. The salt-urea method exploits the stability of the cell nucleus
Figure 5.13.2 Preparation of 6 . (a) 1,2,4‐triazole, TEA, POCl3 , MeCN:DCM (1:1 v/v); (b) sodium salt of 1,7‐heptanediol, dioxane; (c) Pac‐Cl, TEA, THF; (d) TBAF (1 M in THF), THF; (e) Cl‐P(OCE)N i Pr2 , DIPEA
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