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Cajal Body Isolation Protocol

were also a significant contaminant. D. GRADIENT TWO 16. Add 600 units of DNase1 (Sigma Cat no: D4527; 20,000 units/ml) to Fraction 1P. Mix with a rotating wheel for 1 hour at RT. 17. Add 0.05x volume of heparin (10mg/ml). The mixture

Cell Isolation Theory

of animal extracellular connective tissue; skin, tendon, blood vessels, bone, etc. In brief, collagen consists of fibrils composed of laterally aggregated polarized tropocollagen molecules (M.W. 300,000). Each rod-like tropocollagen unit consists

2D Gel Electrophoresis

might come out of solution, be aware of potential protease activity though.Hot SDS can be used to increase initial protein solubilisation. However, before electrophoresis it must be removed by competitive displacement in SD buffer. Post solubilisation TCA