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文献和实验while vortexing. If Biotinylating, add 150uL of Biotin/Water solution to Antibody while vortexing. 3. Incubate at room temperature for 45minutes. 4. The next step is to separate the coupled from uncoupled FITC or Biotin. This can be done in 2 ways
while vortexing. If Biotinylating, add 150uL of Biotin/Water solution to Antibody while vortexing. 3. Incubate at room temperature for 45minutes. 4. The next step is to separate the coupled from uncoupled FITC or Biotin. This can be done in 2 ways
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
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