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上海玉博生物科技有限公司
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文献和实验Materials (Solutions are all available from the media room) 200ml bottle of 2x SD 200ml bottle of 4% agar -- make sure to sign it out 40% glucose CSM minus the relevant marker(s), as powder Stack
ml D. 20×Leu 25.0ml E. Agar 10.00g F. ddH2O → 500.0ml 90-100mm)f铺20-25块平板( 11. 挑取上述固体培养基上生长的含有目的DNA(X)的pLexA-X(+)或空载pLexA(-)单菌落，分别接种SD/Gal/Raf/-Ura，-His，-Trp，-Leu固体诱导培养板，30℃倒置培养3-5天。 SD/Gal/Raf/-Ura，-His，-Trp，-Leu固体诱导
of the assay, which is simply done on agar plates, is that if the experimental strain, which should have at least one of the more common auxotrophic markers (e.g., trp1 or ura3), is mated to the mating tester strains, any resulting diploids will be able to grow
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