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上海玉博生物科技有限公司
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文献和实验ml D. 20×Leu 25.0ml E. Agar 10.00g F. ddH2O → 500.0ml 90-100mm)f铺20-25块平板( 11. 挑取上述固体培养基上生长的含有目的DNA(X)的pLexA-X(+)或空载pLexA(-)单菌落,分别接种SD/Gal/Raf/-Ura,-His,-Trp,-Leu固体诱导培养板,30℃倒置培养3-5天。 SD/Gal/Raf/-Ura,-His,-Trp,-Leu固体诱导
Materials (Solutions are all available from the media room) 200ml bottle of 2x SD 200ml bottle of 4% agar -- make sure to sign it out 40% glucose CSM minus the relevant marker(s), as powder Stack
Two-hybrid analysis of genetic
combinations of strains,plasmids,and reporters can be used for mating (Section 3).In one common version (Finley and Brent,1994),the strain expressing the bait (bait strain)is RFY206 (MATa ura3-52 his3Æ200 leu2-3 lys2Æ201 trp1::hisG)transformed with the HIS3
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