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文献和实验Cryopreservation and Recovery of Mature Differentiated Neural Cells
实验试剂 Neurobasal® Medium B-27® Serum-Free Supplement GlutaMAX™-I Trypan Blue Synth-a-Freeze® Cryopreservation Medium 实验设备 Cryogenic Vials Isopropanol Chamber
Differentiating Neural Stem Cells into Neurons and Glial Cells
实验试剂 KnockOut™ D-MEM/F-12 Dulbecco’s Modified Eagle Medium (D-MEM) Dulbecco’s Phosphate-Buffered Saline (D-PBS) StemPro® NSC SFM N-2 Supplement B-27® Serum-Free Supplement Neurobasal®
Slice and Explant Culture Protocols Hevner lab 2002
100 ml of sterile filtered Krebs buffer and cool on ice. 3. Prepare 100 ml serum�supplemented medium, and place in incubator set at 37°C and 5% CO2. 4. Prepare 100 ml serum�free medium, and place in incubator set at 37°C and 5% CO2. b. 30 min
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