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上海玉博生物科技有限公司
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文献和实验Intracellular Cytokine Staining Protocol
-gamma, TNF-alpha, IL-2, and IL-4, a combination of PMA (a PKC activator) and Ionomycin (a calcium ionophore) or anti-CD3 antibodies are used. To induce IL-6, IL-10 or TNF-alpha production by monocytes, stimulation of PBMC's with LPS is used.Note: Optimal
Intracellular Cytokine Staining Protocol
to produce IFN-gamma, TNF-alpha, IL-2, and IL-4, a combination of PMA (a PKC activator) and Ionomycin (a calcium ionophore) or anti-CD3 antibodies are used. To induce IL-6, IL-10 or TNF-alpha production by monocytes, stimulation of PBMC's with LPS is used
Transplantation Models to Characterize the Mechanisms of Stem Cell–Induced Islet Regeneration
antibodies: FITC‐labeled anti‐human CD45 antibody (4 µl/200 µl cells; BD Biosciences, cat. no. 555482)
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