Cryopreservation and Recovery of Mature Differentiated Neural Cells
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实验试剂
Synth-a-Freeze® Cryopreservation Medium
实验设备
实验材料
实验步骤
2) Count the cell number using a hemocytometer.
3) Centrifuge the cells at 200 × g for 4 minutes. Aspirate the supernatant.
6) Transfer the isopropanol chamber to -80°C for overnight.
7) Transfer the frozen vials to the vapor phase of liquid nitrogen storage until use is required.
1) Remove one vial of frozen cells from liquid nitrogen.
2) Thaw the vial in a 37°C water bath with gentle swirling.
4) Open the vial in a laminar flow hood.
8) Mix the suspension very gently with P-1000 pipette. Avoid creating any air bubbles.
11) Incubate the cells at 37°C in a humidified atmosphere of 5 % CO2 in air.
