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文献和实验Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
. B.2.2. Methodology 1. Tissue sections are dewaxed and rehydrate by heating at 60°C. 2. Wash in xylene and rehydrate through a graded series of ethanol and deionized water. 3. Stripp from proteins by incubation with 20 m g/mL
or other suitable peroxidase substrate. 21. Wash thoroughly in running tap water. 22. Counterstain with haematoxylin (if required), dehydrate and mount. SOLUTIONS 1. TRYPSIN SOLUTION 1. Preheat the following to 37oC using a water bath: (i) 200ml of TBS
to distilled water. 3. Place sections in 0.5% hydrogen peroxide/methanol for 10 minutes (or use other appropriate endogenous peroxidase blocking procedure). Wash sections in tap water. 4. Heat 1500ml of the recommended unmasking solution (0.01M citrate
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