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文献和实验Silver Staining Protocol for Acrylamide Gels
) 453 gms 10X TBE 50 ml 40%(19:1 ratio) Acrylamide : bisacrylamide
SSR GEL and Silver Staining Protocol
8 cm, and one lid Two rocking platforms Heat block for microtiter plates. A microplate vortexer is helpful. II. MOLD ASSEMBLY: Notes: Bind silane is toxic and should be used in a chemical fume hood. Wear gloves when handling this solution. Use
SSR GEL and Silver Staining Protocol
before working with bind silane and take care not to cross contaminate the plates with the two treatments. The acrylease treatment only needs to be repeated every four gels or so. 3. Prepare fresh 1 ml binding solution by making a solution 0.0005-0.001% bind
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