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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
, during time series analyses or when screening transfor-mants for expression of a transformed gene. Under such circumstances, existing tech-niques are overly time consuming and yield more RNA than is necessary. The availability of a rapid RNA mini-prep
A quick RNA mini-prep for Neurospora mycelial cultures
10. Spin at 12000g, 4°C, for 15 min in a fixed angle rotor. Thoroughly resuspend pellet in 4 ml sterile gd (glass distilled) H2 O with pasteur pipette. 11. Extract twice with phenol/chloroform/isoamyl alcohol (25:24:1), spinning 12000g 10 min
ORNL MICROARRAY HYBRIDIZATION PROTOCOLS
Technologies; Cat # 48190-011) Cyanine 3-dUTP # NEL578 or Cyanine 5-dUTP #NEL579 (Perkin Elmer/NEN) B. REAGENT PREPARATION 50X dNTPs 50X [ ] Stock [ ] For 1.0 ml of 50X use:
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