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文献和实验Construction and Characterization of Adenovirus Vectors
by filtration through a 0.2-µm filter. Sucrose (40% w/v, in PBS-Ad Sterilize by filtration through a 0.2-µm filter. For 4% sucrose, dilute the stock solution with PBS-Ad and sterilize by filtration before use. TE-Ad (1X) For 0.1X TE-Ad
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
) (for Protocols 2-4) Recombinant murine IL-7 (Peprotech 217-17) (for Protocols 2-4) Trypsin solution, 0.25% (Invitrogen 15090046) (for Protocols 1, 2) Dilute stock trypsin to 0.25% with PBS. Equipment Biosafety cabinet Cell strainers (40-µm pore size
Lacrimal Experimental Protocol (4/4/94)
µl of 1 M stock FBS 3 ml (heat deactivated) DMEM/gent. 25.5 ml (high glucose) *add just before use 7. Prepare DMEM/STI/20% FBS by mixing 40 ml of DMEM/STI/gent. with 10 ml of FBS. 8. Perfuse (3) male 4 wk Sprague Dawley rats (from NCI) with DMEM
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