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文献和实验Western blotting using ECL reagent
membrane (Amersham Life Science; cat # RPN2020D)Kodak X-OMAT (XAR-5, 18X24 cm; cat # 8532665)10X TBS12.11 g Tris-base (100 mM)87.66 g NaCl (1500 mM)1 liter dd H2OAdjust pH= 7.6Washing buffer (TBS-T): 100 ml 10X TBS + 900 ml dd H2O + 1 ml Tween-20.Blocking
the 0.5% sodium hydroxide and hydrochloric acid solutions. Return the trough to the water bath and allow this enzyme solution to re-heat to 37°C (See note iv). Transfer the warmed slides into the enzyme solution for a suggested 20 minutes (See note v
IMMUNOHISTOCHEMISTRY on PARAFFIN OR CRYOSECTIONS
dH2O 2 mL unmasking solution a) Fill a rectangular dish 1/3 full with dH20. This will be used as a water bath for the glass jar containing the diluted unmasking solution. Place glass jar containing above solution in the bath and heat in microwave
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