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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
Protease inhibitor cocktail for ChIP (1000X) Proteinase K (20 mg/mL) (Roche Diagnostics) Reagents for agarose gel electrophoresis RIPA buffer for ChIP RNase A SDS (sodium dodecyl sulfate) (10%) T cell isolation buffer
of a 50% slurry of Glutathione Sepharose 4B (prepared as described above)to each supernatant and mix gently for 5 min at r.t..Add 100 μl of 1 x PBS,vortex briefly,and centrifuge for 5 sec to sediment the Sepharose beads.Discard the supernatant,repeat
making it possible to use the technique in E. coli and other commonly used bacterial strains (10). In addition, the recombination efficiency was increased 10�100 times (11). The combination of these three enzymes (α, β and γ, or E, T and γ) in one vector
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