BD特约代理商|655050 BD FACSDiva CS&

Native Chromatin Preparation and Illumina/Solexa Library Construction

Protease inhibitor cocktail for ChIP (1000X) Proteinase K (20 mg/mL) (Roche Diagnostics) Reagents for agarose gel electrophoresis RIPA buffer for ChIP RNase A SDS (sodium dodecyl sulfate) (10%) T cell isolation buffer

GST融合蛋白纯化--筛选表达株

of a 50% slurry of Glutathione Sepharose 4B (prepared as described above)to each supernatant and mix gently for 5 min at r.t..Add 100 μl of 1 x PBS,vortex briefly,and centrifuge for 5 sec to sediment the Sepharose beads.Discard the supernatant,repeat

Assisted large fragment insertion by Red/ET-recombination (ALFIRE)―an alternative and enhanced method for large fragment recombineering

making it possible to use the technique in E. coli and other commonly used bacterial strains (10). In addition, the recombination efficiency was increased 10�100 times (11). The combination of these three enzymes (α, β and γ, or E, T and γ) in one vector