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文献和实验Propagation, Quantification, Detection, and Storage of West Nile Virus
. Daffis, S., Lazear, H.M., Liu, W.J., Audsley, M., Engle, M., Khromykh, A.A., and Diamond, M.S. 2011. The naturally attenuated Kunjin strain of West Nile virus shows enhanced sensitivity to the host type
Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
for the pre-HYB and post HYB washes: Prepare 1 liter of HYB buffer omitting the DNA. Aliquot HYB into 4 bottles as follows: - 400ml in one bottle labeled HYB w/DNA. Add 4ml of 10mg/ml ssDNA to that aliquot. - 360ml in one bottle labeled HYB wash
of Huh7 cell maintenance medium. Using a multichannel pipettor, three 1:10 dilutions can be made by transferring 20 µl between wells down each column, beginning with the undiluted virus row and moving on to the 1:10 row, the 1:100 row, and the 1:1000 row
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