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上海玉博生物科技有限公司
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文献和实验sequence pre-filled out. See Ambion's Custom siRNA Oligonucleotide Synthesis Service for details. **Template sequences for the Silencer™ siRNA Construction Kit. References 1. Harborth J, Elbashir SM, Bechert K, Tuschl T, and Weber K. (2001
The following protocol is for MEGAscript II Kit (Ambion). 1. PCR amplify the DNA template. The 5'-end of the template should contain the minimum promoter sequenences of T7 or Sp6 or T3. A 5-primer with the minimum
The following protocol is for MEGAscript II Kit (Ambion). 1. PCR amplify the DNA template. The 5'-end of the template should contain the minimum promoter sequenences of T7 or Sp6 or T3. A 5-primer with the minimum promoter sequences may be used
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