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文献和实验Denaturing Agarose Gel Electrophoresis of RNA
, such as those from needle biopsies or from laser capture microdissected samples, result in very low yields. In these cases, it may be impossible to spare 200 ng of RNA to assess integrity. Alternative nucleic acid stains, such as SYBR® Gold and SYBR® Green II RNA gel stain
between crystals of protein?nucleic acid complexes and those containing protein alone is a common problem in structural studies of protein?nucleic acid interactions. Currently, there are several methods available for detecting nucleic acid in crystals, including
Protocol for competitive RT-PCR
, stained with ethidium bromide, SYBR-Green or SYBR-Gold (Molecular Probes, higher sensitivity, but also more light sensitive) and scanned by a CCD camera. The amount of cDNA used for a PCR, the number of cycles and the nucleic acid stain used depend
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