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上海玉博生物科技有限公司
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文献和实验Purpose The protocol describes how to prepare human tonsil lysate for use in purification of ICAM-1,LFA-1,and PNAd. Materials Safety Equipments Lab Coat Latex Gloves Face Mask Bench Paper Fresh human tonsil tissue Parafilm Scissors
RNA Purification Protocol for 0.1-2.5 mg of Animal Tissue
(Product # SSI-1015-39). 3. Add 33μl of PNP Buffer to the cell lysate. Mix the sample gently by inverting the tube 10 times. 4. Place the tube on ice for 5 minutes. 5. Centrifuge at max speed (?13,000 x g) for 3 minutes at room
RNA Purification Protocol for 100-200 mg of Animal Tissue
strokes with tube pestle. 3. Add 2 ml of PNP Buffer to the cell lysate. Mix the sample gently by inverting the tube 10 times. 4. Place the tube on ice for 5 minutes. 5. Centrifuge at 3,000-5,000 g for 10 minutes at room temperature
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