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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
histochemical reporter gene (Beckwith, 1980). It can be detected using a variety of substrates, all of which have galactose linked through a ß-D-glycosidic linkage to a moiety whose properties change upon liberation from galactose (Wallenfels and Weil, 1972
. Stop reaction as recommended by substrate manufacturer. Alternatively, for peroxidase substrates, stop with deionized water. For phosphatase substrates, stop with either 50 mM Na2-EDTA or deionized water. Air dry membrane. 15. Store membrane long
with TS for 15 minutes, once with TSN for 15-20 minutes and finally once with TS for 15 minutes. 8. Develop color using the appropriate substrate: For HRP conjugates, mix 5 parts TMB peroxidase substrate, 5 parts TMB solution B, and 1 part TMB membrane
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