thermo特约代理商|31505 Mouse IgG Is

Isotype-specific immunostaining with mouse primary antibodies on mouse tissue

. A cheap alternative is to exploit the relative lower levels of each IgG isotype in serum and on follicular B cells to stain with isotype-specific, conjugated, anti-mouse secondary antibodies. IgG isotypes account each for 20-25% ot total serum

Rapid Isotyping of Mouse Monoclonal Antibodies

for 5% of mouse antibodies) has limited practical value. In contrast, the heavy-chain isotype is very informative (see Table 1 ); protein A or protein G binding, euglobulin properties, complement binding, kinetics of digestion with papain or pepsin, clearance

FITC荧光二抗？――EarthOx新型DyLight 488绿色荧光

的价格比常规二抗 只高20%左右，但是Dylight的效价通常是常规荧光二抗的2-4倍左右，因此就最终的性价比，Dylight更胜一筹。举例来说，EarthOx的 FITC标记的山羊抗小鼠IgG二抗（货号为E031210），100μl和500μl报价分别是￥160和￥640；而相应的EarthOx的 Dylight 488标记的山羊抗小鼠IgG二抗（货号为E032210），100μl和500μl报价分别是￥200和￥800，但是在具体使用时，比如免疫荧光实验，其推荐稀释比率分别为1:100