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文献和实验【求助】5'-deoxyribose-5-phosphate(5'-dRP) lyase以及5'-dRP/AP lyase的概念
, R., Beard, W. A., Strauss, P. R. & Wilson, S. H. Human DNA polymerase β deoxyribose phosphate lyase. Substrate specificity and catalytic mechanism. J. Biol. Chem. 273, 15263–15270 (1998) 3 Sobol, R. W. et al. The lyase activity of the DNA
Characterization of DNA Strand Cleavage by Enzymes That Act at Abasic Sites in DNA
directed toward a modified or nonconventional base in DNA. Some of these N -glycosylases also possess AP lyase activity that cleaves DNA 3′ to an AP site via a β-elimination reaction to leave a 3′ 4-hydroxy-2-pentenal-5-phosphate (Fig. 1 ). An example
Site‐Specific Protein Labeling with SNAP‐Tags
labeling of cellular proteins with chemical probes is a powerful tool for studying protein function in living cells. A number of small peptide and protein tags have been developed that can be labeled with synthetic probes with high efficiencies
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