【求助】5'-deoxyribose-5-phosphate(5'-dRP) lyase以及5'-dRP/AP lyase的概念
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最近看到一篇文章，里面提到了
5'-deoxyribose-5-phosphate(5'-dRP) lyase以及5'-dRP/AP lyase的概念
但是我查了半天也没搞明白这到底是指什么，请各位好朋友指点！！谢谢大家了。。
5'-deoxyribose-5-phosphate(5'-dRP) lyase以及5'-dRP/AP lyase的概念
但是我查了半天也没搞明白这到底是指什么，请各位好朋友指点！！谢谢大家了。。
http://www.brenda-enzymes.org/php/result_flat.php4?ecno=4.2.99.B1
http://www.pnas.org/content/95/21/12244.full
Mammalian cells require non-homologous end joining (NHEJ) for the efficient repair of chromosomal DNA double-strand breaks. A key feature of biological sources of strand breaks is associated nucleotide damage, including base loss (abasic or apurinic/apyrimidinic (AP) sites). At single-strand breaks, 5′-terminal abasic sites are excised by the 5′-deoxyribose-5-phosphate (5'-dRP) lyase activity of DNA polymerase β (pol β).
Excision repair of a damaged base requires the concerted activities of a glycosylase to remove the damaged or inappropriate base, a class II apurinic/apyrimidinic (AP) endonuclease to incise the DNA 5′ to the AP site, a lyase activity to remove the 5′ terminal 2-deoxyribose-5-phosphate (dRP) sugar moiety from the downstream DNA, resynthesis, and ligation
如果还不明白，请参阅：
1 Matsumoto, Y. & Kim, K. Excision of deoxyribose phosphate residues by DNA polymerase β during DNA repair. Science 269, 699–702 (1995)
2 Prasad, R., Beard, W. A., Strauss, P. R. & Wilson, S. H. Human DNA polymerase β deoxyribose phosphate lyase. Substrate specificity and catalytic mechanism. J. Biol. Chem. 273, 15263–15270 (1998)
3 Sobol, R. W. et al. The lyase activity of the DNA repair protein β-polymerase protects from DNA-damage-induced cytotoxicity. Nature 405, 807–810 (2000)
4 Allinson, S. L., Dianova, I. I. & Dianov, G. L. DNA polymerase β is the major dRP lyase involved in repair of oxidative base lesions in DNA by mammalian cell extracts. EMBO J. 20, 6919–6926 (2001)
http://www.pnas.org/content/95/21/12244.full
Mammalian cells require non-homologous end joining (NHEJ) for the efficient repair of chromosomal DNA double-strand breaks. A key feature of biological sources of strand breaks is associated nucleotide damage, including base loss (abasic or apurinic/apyrimidinic (AP) sites). At single-strand breaks, 5′-terminal abasic sites are excised by the 5′-deoxyribose-5-phosphate (5'-dRP) lyase activity of DNA polymerase β (pol β).
Excision repair of a damaged base requires the concerted activities of a glycosylase to remove the damaged or inappropriate base, a class II apurinic/apyrimidinic (AP) endonuclease to incise the DNA 5′ to the AP site, a lyase activity to remove the 5′ terminal 2-deoxyribose-5-phosphate (dRP) sugar moiety from the downstream DNA, resynthesis, and ligation
如果还不明白，请参阅：
1 Matsumoto, Y. & Kim, K. Excision of deoxyribose phosphate residues by DNA polymerase β during DNA repair. Science 269, 699–702 (1995)
2 Prasad, R., Beard, W. A., Strauss, P. R. & Wilson, S. H. Human DNA polymerase β deoxyribose phosphate lyase. Substrate specificity and catalytic mechanism. J. Biol. Chem. 273, 15263–15270 (1998)
3 Sobol, R. W. et al. The lyase activity of the DNA repair protein β-polymerase protects from DNA-damage-induced cytotoxicity. Nature 405, 807–810 (2000)
4 Allinson, S. L., Dianova, I. I. & Dianov, G. L. DNA polymerase β is the major dRP lyase involved in repair of oxidative base lesions in DNA by mammalian cell extracts. EMBO J. 20, 6919–6926 (2001)
谢谢，其实我就是看了那篇关于ku的文章后才不明白的，呵呵~~~我再查查别的资料，感谢你的帮助
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