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文献和实验the beadbound cells with a magnet. Discard the bead-bound cells and use the remaining untouched, enriched cell population for further flow sorting into the DC subpopulation(s) of interest 实验试剂 Magnet: (Dynal® MPC™), MPC-L for 1–5 ml
Techniques for generating human T-cell clones (TCCs) were first described nearly two decades ago (1 , 2 ). This was a direct consequence of the discovery of T-cell growth factor and the subsequent ability to propagate T-cells over extended
) should be performed for optimization. Add 100l of the cell suspension to each well. For each condition, use triplicate wells. Add soluble antibody in 100l to each well. Titrate antibodies for optimal performance in the assay conditions used. If isolated T
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