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文献和实验Rapid Extraction of High Quality DNA from Whole Blood Stored at 4ºC for Long Period
100. Add 10 ml of 1 M Tris, 109.54 gr of sucrose, 1.01 gr MgC12, adjust pH to 8.0 and finally add 10 ml of Triton X-100 to 800 ml of distilled water, and make up to 1 liter with distilled water. Autoclave at 15 p.s.i. for 10 min. Sugars at high
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
type of interest, grown under appropriate cell culture conditions with appropriate medium Cells, bacterial, competent, high-efficiency ( 5 x 109 cfu/µg DNA) XL10-Gold ultracompetent cells (Stratagene) have produced optimal results.
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
3) Discard & wash 3x w/ LSWB 15'' at 60 C (see below) 5) Check filter with counter. If more than 10, proceed to 5), otherwise to 8) 6) Discard and wash 3x w/ HSWB at 60 C 7) Check filter w/ counter. Should be less than 10. 8) Blot dry briefly
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