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Single and Dual Birthdating Procedures for Assessing the Response of Adult Neural Stem Cells to the Infusion of a Soluble Factor Using Halogenated Thy

Introduction Basic Protocol 1: Single Labeling of Actively Proliferating Cells to Analyze the Short‐Term Effect of a Soluble Factor on aNSCs Basic Protocol 2: Dual Labeling of Two Cell Division

Gene Knockout In Murine Embryonic Stem (ES) Cells

vectors and resuspend in 750µl MTPBS Before continuing check feeder plates for confluency, if there is something wrong with the feeder layer it is possible to plate them with the ES cells after the electroporation (it is essential to remove the DMSO

Intraspinal Transplantation of Mouse and Human Neural Precursor Cells

with needle support foot (cat. no. 1772), electrode holder with removable open side clamp (cat. no. 1773), dual small animal stereotaxic platform (cat. no. 902) Tri‐fold paper towels