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上海玉博生物科技有限公司
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文献和实验All-in-One Kit for Immunohistochemical Staining for Tissues with Antibodies
near the tissue sections with a laboratory wipe. Circle each tissue section using the Mini PAP Pen. 2. Proceed immediately to Peroxidase Quenching. Peroxidase Quenching Peroxo-Block™ works efficiently on many tissues, especially
免疫组化技术-Immunohistochemical Protocols
2) or a biotin conjugated (Table2) secondary antibody is used, both at a dilution of 1:500 in BSA diluent for 1 hr, Wash in PBS 3x5 min Incubate in avidin-biotin-peroxidase complex (ABC reagent
• stabilizer soln (10% sodium thiosulfate+0.1N NaOH) • IODO-BEAD™(PIERCE) • Hamilton microsyringe (model 702, 25µl, needle gauge 22S, point style #2) • desalting column (Bio-rad DG-10 etc.) • column stand For procedure 2 • PBS
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