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文献和实验Strep/FLAG Tandem Affinity Purification (SF‐TAP) to Study Protein Interactions
, several methods have been developed to analyze protein?protein interactions under native conditions. One of them, tandem affinity purification (TAP), combines two affinity?purification steps to allow isolation of high?purity protein complexes. This unit presents
METHOD DOTAP Method Resuspend neurons freshly prepared from E18 rat hippocampus in HBSS (Ca++ -Mg++ -free), and adjust to 106 cells per milliliter. In a 15-ml polystyrene conical
melanoma cells drastically reduces luciferase reporter activity to 18% of that in control cells (1) . Direct intratumoral injections are performed in an aggressive melanoma model. B16 melanoma-bearing mice injected with pCI-SAP complexed with lipofectamine
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