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文献和实验Vybrant® DyeCycle Green and Orange Stains
or Vybrant® DyeCycle™ Orange stain from the freezer and allow it to equilibrate to room temperature. Prepare flow cytometry tubes each containing 1 mL of cell suspension in complete media at a concentration of 1 × 106 cells/mL. To each tube
Vybrant® DyeCycle Violet Stain
if media is not desired, however phosphate buffers are not recommended Do not use glass containers with this stain Do not wash or fix cells after staining cells with Vybrant® DyeCycle™ Violet stain Validate flow cytometry instrument
Mag-Bind® Plasmid Mega Magnetic Protocol
. The culture density should reach 3-4 x 109 per mL. It is strongly recommended that an endA negative strain of E.coli be used for routine plasmid isolation. Examples of such strains include DH5α® and JM109® . 2. Harvest the bacterial cells
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