QIAGEN凯杰特约代理商|10243 QIAGEN Gen

Qiagen MIDI protocol

in the Beckman. Promptly pour supernatant into another round-bottom 13 ml polycarbonate tube and re-centrifuge at 20,000 x g for 15 min. While the centrifugation is happening, equilibrate a Qiagen-tip 100 (see next step). 7. To equilibrate the tip

Qiagen MIDI protocol(QIAGEN plasmid Midi试剂盒)

and re-centrifuge at 20,000 x g for 15 min. While the centrifugation is happening, equilibrate a Qiagen-tip 100 (see next step). 7. To equilibrate the tip, put it in a plastic collar and put this assembly into the top of a 50-ml conical polypropylene tube. Add

Miniprep/Qiagen kit

Buffer P1 50 mM Tris-HCl pH 8.0 10 mM EDTA 100 μg/ml RNaseA The buffer and RNaseA can also be ordered from Qiagen separately (catalog numbers 19051 and 19101). Buffer P2 200 mM NaOH 1% SDS Buffer P3