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文献和实验in the Beckman. Promptly pour supernatant into another round-bottom 13 ml polycarbonate tube and re-centrifuge at 20,000 x g for 15 min. While the centrifugation is happening, equilibrate a Qiagen-tip 100 (see next step). 7. To equilibrate the tip
Qiagen MIDI protocol(QIAGEN plasmid Midi试剂盒)
and re-centrifuge at 20,000 x g for 15 min. While the centrifugation is happening, equilibrate a Qiagen-tip 100 (see next step). 7. To equilibrate the tip, put it in a plastic collar and put this assembly into the top of a 50-ml conical polypropylene tube. Add
Buffer P1 50 mM Tris-HCl pH 8.0 10 mM EDTA 100 μg/ml RNaseA The buffer and RNaseA can also be ordered from Qiagen separately (catalog numbers 19051 and 19101). Buffer P2 200 mM NaOH 1% SDS Buffer P3
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