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Cell Isolation Theory

to as clostridiopeptidase A which is a protease with a specificity for the X-Gly bond in the sequence Pro-X-Gly-Pro, where X is most frequently a neutral amino acid. Such sequences are often found in collagen, but only rarely in other proteins. While many proteases

Liposome-Mediated, Fluorescence-Based Studies of Sphingolipid Metabolism in Intact Cells

Our laboratory has been engaged in studies of lipid hydrolases since the early 1960s. In the course of these studies the following enzymes have been isolated and partially purified: ceramidase (1 –3 ); two sphingomyelinases: the lysosomal

A Method for Assaying Deubiquitinating Enzymes

). The purified Ub-specific protease-6 (yUBP-6) in yeast and cUBP41, Ub C-terminal hydrolase-1 (cUCH-1), cUCH-6, and cUCH-8 in chick skeletal muscle were prepared as described (13 , 15 -18 ). Ub-PESTc was purified from an E. coli strain AR13 carrying pNMHUB-PESTc