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文献和实验of containing most of the necessary reagents pre-packaged in RNase-free quality （e.g. polyATtract from Promega or Oligotex mRNA kit from Qiagen work well for Neurospora, Table 1）。 Depending on the kit size, this allows isolation of poly（A）-RNA from 0.25 to 5 mg
Dynabeads® mRNA DIRECT Micro Kit - mRNA isolation for RT-PCR amplification
实验原理 Use the Dynabeads® mRNA DIRECT™ Micro Kit for simple and rapid isolation of mRNA from small cell and tissue samples, for direct use in reverse transcription PCR. The direct mRNA isolation is completed in only 15
mRNA Amplification with T7 RNA Polymerase
Materials MessageAmp II aRNA Amplication Kit (Cat #1751, Ambion) 100% Ethanol RNA samples (e.g. 1 µg RNA per sample) Procedure Reverse transcription Verify that EtOH (24 mL) has been added to the Wash Buffer. Turn
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