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上海玉博生物科技有限公司
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文献和实验Rhodamine-Phalloidin/Calcofluor Staining
4 + 2.4 grams KH2PO4. pH upon dilution to 1X should be 7.2. Rhodamine Phalloidin Molecular Probes Inc., Dissolved in methanol according to manufacturers instructions to ~6.6uM and stored dessicated at -20¡C. Cat#R-415. Calcofluor aka
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells
also works well for some antibodies. Generally for actin filaments and the actin cytoskeleton we prefer methanol over acetone fixation, and formaldehyde over glutaraldehyde fixation. Glutaraldehyde requires a reducing step that can mechanically dislodge
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells
also works well for some antibodies. Generally for actin filaments and the actin cytoskeleton we prefer methanol over acetone fixation, and formaldehyde over glutaraldehyde fixation. Glutaraldehyde requires a reducing step that can mechanically dislodge
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