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文献和实验RNAqueous® MAG-96 and then converted to cDNA. Target cDNA levels were analyzed in duplicate using a TaqMan Gene Expression Assay for survivin (Hs00153353_m1). Data shown here represent duplicate assays from single transfections. Survivin mRNA levels
Assay (Invitrogen) as suggested by Illumina. Illumina Bead Array MicroRNA Detection Starting with 500 ng/sample of total RNA, mature microRNAs were amplified with the Illumina human v1 MicroRNA expression profiling kit containing primers for 743
and Desjardins, ), RNA purification by purification kit (Phenol‐Free Total RNA Purification Kit or Ribozol Plus RNA Purification Kit; AMRESCO Inc., http://www.amresco‐inc.com/), lithium chloride precipitation (Diaz‐Ruiz and Kaper, ), spin‐column
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