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文献和实验Dual- and Triple-Color Fluorospot
labeled with green fluorophore emitting light at the same wavelength as FITC, streptavidin (SA) labeled with Cy3, and if three cytokines are analyzed, a mouse anti-tag mAb labeled with Cy5 (Mabtech). 5. Mouse mAb anti-human CD28 at 0.1 mg/ml
Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
from invertebrates to lower and higher verterbrates, and intervenes both in physiological and in pathological states, such as normal cell turnover, embryogenesis, metamorphosis, thymic maturation and involution, neoplasm, etc. (1-4). A mouse anti-DNA monoclonal
Characterization and Validation of Cre‐Driver Mouse Lines
(1:8000 dilution, VWR, cat. no. 69050‐3) was used with goat anti‐rabbit antibody coupled to horseradish peroxidase (1:100 dilution; Invitrogen, cat. no. G‐21234) as secondary antibody. After washing, Cre was visualized by FITC‐tyramide amplification (1/50
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