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文献和实验36 x 0.2ml Standard Micro-tubes 72 x 0.1ml Strip tubes Heating/Cooling Rate: 2.5°C/second (tube temperature) 5.0°C/second (air temperature
(BD Biosciences / Clontech). SYBR Green PCR master mix, 200 reactions (Applied Biosystems). Optical tube and cap strips (Applied Biosystems). SuperScript
. The second step will be to determine how much of each DNA to put into a PCR tube. We are going to be doing 20 ul reactions and with all of the other ingrediants, there is room to put only 8.4 ul of DNA into each tube. If possible I would like you to have 100
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