ScyTek特约代理商|TBT500 Tris Buffer

Western Blotting with Biotinylated Antibodies

for colorimetric detection, visualize the transferred proteins by staining the membrane for 15 minutes with India ink (Higgins black India ink, Eberhard Faber) diluted 1:1000 in wash buffer (10mM Tris pH 7.5, 100mM NaCl, 0.1% Tween 20). Rinse excess stain with wash

常用试剂配制－2

  Acetone / Formaldehyde Fixative Acrylamide Gel, denaturing Acrylamide Gel, nondenaturing Alkaline Phosphatase Buffer 1 (Glycine Buffer, 0.1 M, pH 10.4) Alkaline Phosphatase Buffer 2 (Tris-HCl, 100 mM, pH

Western Blotting with Horseradish Pe

for colorimetric detection, visualize the transferred proteins by staining the membrane for 15 minutes with India ink (Higgins black India ink, Eberhard Faber) diluted 1:1000 in wash buffer (10mM Tris pH 7.5, 100mM NaCl, 0.1% Tween 20). Rinse excess stain with wash