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文献和实验Western Blotting with Biotinylated Antibodies
for colorimetric detection, visualize the transferred proteins by staining the membrane for 15 minutes with India ink (Higgins black India ink, Eberhard Faber) diluted 1:1000 in wash buffer (10mM Tris pH 7.5, 100mM NaCl, 0.1% Tween 20). Rinse excess stain with wash
Acetone / Formaldehyde Fixative Acrylamide Gel, denaturing Acrylamide Gel, nondenaturing Alkaline Phosphatase Buffer 1 (Glycine Buffer, 0.1 M, pH 10.4) Alkaline Phosphatase Buffer 2 (Tris-HCl, 100 mM, pH
Western Blotting with Horseradish Pe
for colorimetric detection, visualize the transferred proteins by staining the membrane for 15 minutes with India ink (Higgins black India ink, Eberhard Faber) diluted 1:1000 in wash buffer (10mM Tris pH 7.5, 100mM NaCl, 0.1% Tween 20). Rinse excess stain with wash
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