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- 2025年07月15日
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文献和实验Clean-up Spin Protocol For DNA fragments (30bp-100 bp) 1) Add 3 x volume of buffer P3, 3 x volume of isopropanol to the enzymatic reaction and mix throughly with pipetting or vortex. The maximum volume of the reaction can be processed
Materials For purifying plasmid DNA from Escherichia coli cells, the Qiagen Spin Miniprep Kit produces quite reliable results. Do not autoclave solutions containing isopropanol or MOPS; use sterile filtration if necessary.
, amphibian, insect and plant origins. For many applications elution of the mRNA from the beads is not required as the beads do not interfere with downstream enzymatic reactions. The bead-bound oligo (dT)25 can also function as a primer for RT
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