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文献和实验A protocol for cleaning and reusing the large 25 x 25 cm plates
We regularly reuse our large 25x25 cm plating trays; initially, however, we were plagued by gross microbiological contamination when reusing the trays. Various combinations of cleaning with a laboratory dishwasher, ethyl alcohol, and UV
the plates in heat sealable bags with paper towels moistened with 3X SSC and seal the bag with a heat sealer. The high external humidity within the sealed bag helps keep the volumes in the individual wells from varying. 3. Place the bags in a 65
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
, freeze in lN2, may store. 9) Batch adsorb w/ 4ml 50% slurry GT-Sepharose (PL 17-0756-01), 1hr, 4 C, spin 2'', 4k on bench. 10) aspirate, resuspend in 25ml HBT, spin, repeat. 11) pour slurry into column (Econo 1.7x20), elute
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