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文献和实验so it is better to carefully aspirate off the supernatant rather than pour it off. 10. Air dry pellets at room temp. When the DNA pellets turn from while to translucent in appearance, i.e., when most of the ethanol has evaporated, resuspend each in 40 ul TE
is separated from the DNA by loading the solution onto an equilibrated ion exchange column. The A260 containing fractions are pooled, diluted, and ethanol precipitated, and the final DNA pellet is resuspended in buffer and assayed by restriction digestion
. 9) Spin 10 minutes at 7,000 rpm in SS34 rotor. 10) Pour off supernatant and resuspend cells in 10% glycerol, using a volume of 2.0 ml per liter of initial culture. 11) Aliquot to microfuge tubes (100-200 µl per tube) and freeze quickly in a dry ice-ethanol
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