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文献和实验DpnI mediated site-directed Mutagenesis
the amplification to work efficiently with pfu take a bit of fiddling, amplification with Ex taq appears very robust for large plasmids. REFERENCES Fisher, C. L., and Pei, G. K. (1997). Modification of a PCR -based site-directed mutagenesis method
Proteoglycan: Site Mapping and Site-Directed Mutagenesis
flanked by a C-terminal glycine and proximal N-terminal acidic amino acids; however, not all simple Ser-Gly motifs constitute a modification site. Here we present a rapid method for cloning small, defined segments of putative proteoglycan attachment sites
Site-directed Mutagenesis using PCR
From: Molecular Biology: Current Innovations and Future Trends. Eds. A.M. Griffin and H.G.Griffin. ISBN 1-898486-01-8 1995 Horizon Scientific Press, PO Box 1, Wymondham, Norfolk, U.K.In vitro site-directed mutagenesis is an invaluable technique
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