click chemistry tools特约代理商|104

The Polymerase Chain Reaction (PCR)

construction and screening could take many months and libraries had to be made for each individual analysed. The Invention of PCR　　• Invented by Kary Mullis in 1983.　　• First published account appeared in 1985.　　• Awarded Nobel Prize for Chemistry in 1993

Nucleoside Modification with Boron Clusters and Their Metal Complexes

for the synthesis of nucleosides modified with borane clusters and metallacarborane complexes are presented. These include: (1) the click chemistry approach based on Huisgen 1,3?dipolar cycloaddition and (2) tethering of the metallacarborane group to the aglycone

Two-colour fluorescent in situ DNA hybridization on whole mount Drosophila embryos and larval imaginal discs

Purify through the purple Qiagen column; Elute with 50µl of ddH2 O (Sigma); Concentrate the probe by drying, using a speed-vaac until about 2-5µl are left (do not overdry); Resuspend the labeled probe in the FISH Hybridization Buffer