click chemistry tools特约代理商|104

NAR新突破：CliPEdit调控RNA功能与m⁶A甲基化，翌圣试剂助力

试剂 BANEIC（含双叠氮基团与光裂解硝ji苯基团）和 t-DBCO（三价DBCO交联剂），通过三步调控实现 ON → OFF → ON 的RNA功能切换： Step1. ON 态：BANEIC修饰RNA：使其同时具备—点击化学（click chemistry）可连接性（含叠氮基）、光可裂解性（含硝ji苯基团）。修饰后 gRNA 功能正常，靶向写入 m⁶A； Step2. OFF态：加入 t‑DBCO，通过 SPAAC 点击反应交联 gRNA，空间位阻使其彻底失活，m⁶A甲基化关闭； Step

The Polymerase Chain Reaction (PCR)

construction and screening could take many months and libraries had to be made for each individual analysed. The Invention of PCR　　• Invented by Kary Mullis in 1983.　　• First published account appeared in 1985.　　• Awarded Nobel Prize for Chemistry in 1993

Nucleoside Modification with Boron Clusters and Their Metal Complexes

for the synthesis of nucleosides modified with borane clusters and metallacarborane complexes are presented. These include: (1) the click chemistry approach based on Huisgen 1,3?dipolar cycloaddition and (2) tethering of the metallacarborane group to the aglycone