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Blocking cDNA arrays printed on amine slides

into each other while shaking. Prepare BLOCKING SOLUTION: Have three 350 ml glass chambers available (with metal tops) and a large round pyrex dish with dH2O ready in the microwave. At this time, prepare the 15ml sodium borate

Making Boiled Donkey (or whatever) Serum (BDS) for Blocking

at a 5-10% serum solution. The last batch, for example, worked at 6%, but the next batch worked at 3%.   Fresh 4% Formaldehyde DO ALL STEPS IN THE HOOD In 10mls ddH2 O, add 0.8 g paraformaldehyde Bring to 80oC in 50 ml conical tube

原创：western protocol

1.5M Tris(PH 8.8) 100ml 18.171g (at4°C) 10% SDS 100 ml 10g (at room temperature) 10% ammonium persulfate 1ml 0.1g(replace every week store at at4°C) TEMED 8 room temprature Antibodies Primary antibody: 5A11(anti-mouse IgG) 500x-1000x Secondary