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- Boston biochem
- E-305
- 2025年07月11日
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文献和实验Studies of the Ubiquitin Proteasome System
and Purification of Anti‐Ubiquitin Antibodies Support Protocol 3: Preparation of Ubiquitin Affinity Column Generation of Reagent‐Grade Ubiquitin Activating Enzyme (E1) Basic Protocol 20: Preparation of Wheat E1 in E. coli Basic Protocol 21: Preparation of Mouse E1
(Boston Biochem) Recombinant USP14 (see protocol 5 or purchase commercially from Boston Biochem) Ubiquitin, bovine (Sigma
Stability and Ubiquitination of the Tumor Suppressor Protein p53
-dependent manner through its covalent thio-ester linkage to the E1 ubiquitin-activating enzyme. The activated ubiquitin is then transferred to the E2 enzyme, also known as a ubiquitin conjugating enzyme (UBC), again in the form of a high-energy thio-ester
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