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文献和实验How to Make Simple Solutions and Dilutions
. (stock solution attributes) V1C1=V2C2 (new solution attributes) Example: Suppose you have 3 ml of a stock solution of 100 mg/ml ampicillin (= C1) and you want to make 200 ul (= V2)of solution having 25 mg/ ml (= C2
Replication timing by density transfer
. (I add 0.1 mg/ml ampicillin if I'm feeling paranoid.) The doubling time of RM14-3a is ~ 2.6 hr at 23o C in glucose and 6-8 hr (depending on aeration) in acetate--longer if you're selecting for a plasmid. RM14-3a is MATa, cdc7-1ts , bar1, trp1-289
- 2.6% - Collagen - - - - 1.6 mg/ml - - 0.1N NaOH - - - - 0.12% - - HEPES - - - - 10% - - 7.5% Sodium bicarbonate - - - - 0.8% - - PureCol - - - - 50%
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