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- 2025年07月13日
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文献和实验Using a Counting Chamber For microbiology, cell culture, and many applications that require use of suspensions of cells it is necessary to determine cell concentration. One can often determine cell density of a suspension
Cell counting with an hemacytometer.
). Carefully and continuously fill evenly at once the hemacytometer chamber. Leave undisturbed for 1-2 min. (If you need to leave for longer, use a humid chamber). This will allow deposition of the cells on the counting plane. Count under the microscope
4) Centrifuge on 4 for 5 minutes. Aspirate supernatant and flick pellet. 5) Add 37°C KCl (0.559 g KCl in 100 ml water) drop by drop, flicking the pellet with each drop for the first 10-15 drops. Bring volume up to 7 ml, invert several times, and incubate
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